Preparing 6mL of 0.1M glutathione solution with each 184mg vial
The high–quality support consists of glutathione that has been immobilized by its central sulfhydryl group via a 12–atom spacer arm to crosslinked 6% beaded agarose. Purification of GST–fusion proteins using glutathione (GSH) agarose beads is well documented and adaptable to a variety of scales, column formats and specific applications. Whether the purpose is to purify large amounts of recombinant protein from over–expressing E. Coli lysates or to investigate protein interactions involving GST–tagged bait proteins, Pierce Glutathione Agarose is suitable for the task.
To accommodate these many uses, Pierce Glutathione Agarose is offered in several package sizes and formats. These include three volumes of resin slurry, three sizes of centrifuge-ready columns, complete GST purification kits, and two sizes of FPLC–ready chromatography cartridges.
High capacity – binds at least 25mg of recombinant GST protein per milliliter of resin
High yield and purity – consistently purifies at least 10mg of GST – tagged protein per milliliter of resin with greater than 90% purity
Cost-effective – resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance
Versatile – works well to purify GST – fusion proteins from bacterial lysates or use with pre-purified GST – tagged proteins to pull down protein interactions
Compatible – validated and effective for use with Thermo Scientific Pierce Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures
Flexible – available in multiple formats including bulk resin, spin columns, chromatography cartridges and convenient GST purification kits