Thermo Scientific™ Pierce™ FITC and Fluorescein Dyes and Labeling Kits
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Brand: Thermo Scientific™ 53029
Code : PN
Additional Details : Weight : 1.81440kg
DescriptionFluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.
- Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
|Pierce Fluorescein Antibody Labeling Kit|
|Borate Buffer, 1mL|
|Multi-component kit with fluorescein fluorescent dye|
|Labeling and purifying 3 x 1mg (2mg/mL) of IgG or other proteins|