Thermo Scientific™ Pierce™ NHS-Fluorescein Antibody Labeling Kit

Product Code.	Quantity	unitSize
10628664	3 reactions kit	1 set

Product Code. 10628664 Supplier Thermo Scientific™ Supplier No. 53029

Description

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

• Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery

Specifications

• Description: Pierce Fluorescein Antibody Labeling Kit
• Product Type: Antibody Labeling Kit
• Quantity: 3 reactions kit
• Sufficient For: Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
• Content And Storage: Sufficient For: Labeling and purifying 3 x 1 mg (2 mg/mL) of IgG or other proteins
  NHS Fluorescein, 3 x 50 μg vials
  Borate Buffer, 1 mL
  Purification Resin, 5 mL
  Spin Columns, 6 each
  Collection Tubes, 12 each
  
  Store NHS-Fluorescein at -20°C. Store other kit components at 4°C.
• Excitation/Emission: 494/518
• Labeling Scale: 0.5 to 1 mg
• Product Line: Pierce
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
• Label or Dye: FITC (Fluorescein)
• Labeling Target: Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.