Tandem Mass Tag™ (TMT™) Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. The iodoTMT™ Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (-SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S–nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.
Specific – only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
Irreversible – labeled proteins and peptides are not susceptible to reducing agents
Flexible – options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
Versatile – can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)
IodoTMT Products replace our previously offered CysTMT Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls.