missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ ConA and WGA Glycoprotein Isolation Kits

Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.

Brand:  Thermo Scientific™ 89805

UNSPSC : 41106510

Code : PN

Additional Details : Weight : 0.10000kg

 View more versions of this product

Product Code. 10290154

  • £257.00 / 1 set


EU Stock 4
Add to basket

Specifications

Description

These two kits use lectins concanavilin A (ConA) and wheat germ agglutinin (WGA), respectively, as ligands to bind specific classes of glycoproteins from complex protein samples. ConA lectin recognizes alpha-linked mannose and terminal glucose residues, while WGA lectin selectively binds to N-Acetyl glucosamine (GlcNAc) groups and to sialic acid. The kits include ConA or WGA beaded agarose resin, optimized binding/wash and elution buffers, and easy-to-use microcentrifuge spin columns. The kits have been used to successfully isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.

Highlights:

  • High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers

  • Fast – glycoprotein purification in less than one hour

  • Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate

  • Robust – lectin does not leach from resin when processing sample

  • Convenient – complete kits include lectin resins and spin columns with all necessary reagents

  • Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Includes:

ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.

Description & Specifications

Specifications

Multi-component kit with wheat germ agglutinin agarose resin
Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640uL (1 to 1.5mg total protein) each
10 rxn kit
Binding/Wash Buffer (5X), 6.5mL
Pierce Glycoprotein Isolation Kit, WGA
Agarose
Documentation

Technical Documents