Thermo Scientific™ Pierce™ Agarose ChIP Kit

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 26160

PROTEINASE K, 0,25 ML - 20 MG/ML

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: Weight: 0.00020kg



Product Code. 10504885

Quantity Price
1 £ 87.75 / Each
EU Stock 1
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Description and Specification

Specification

Description Pierce Proteinase K
Formulation Proteinase K diluted to 20mg/mL from stock with an activity of >/= 30U/mg at 35deg.C
Quantity 0.25mL
Sufficient For Use in approx. 30 typical ChIP assays

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

  • Simple and streamlined ChIP assay protocol (< 8 hours)
  • Highly efficient isolation and lysis of nuclei
  • Easy and reproducible enzymatic digestion
  • Low-background and high-capacity Protein A/G agarose resin
  • Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
  • Fast and reproducible spin-column format; columns and collection tubes included
  • High-recovery DNA purification
  • Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls