Thermo Scientific™ Pierce™ Agarose ChIP Kit

Product Code. 10220425

Brand: Thermo Scientific™ 26156

Description

Chromatin immunoprecipitation (chromatin IP, ChIP) assay kit containing all reagents, agarose affinity beads, and a simple, fast, reliable protocol.

Thermo Scientific™ Pierce Agarose ChIP Kit provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

This chromatin IP kit contains sufficient reagents to perform 30 ChIP assays with appropriate controls using an optimized protocol and a convenient spin-column format. The kit includes a complete Chromatin Prep Module (also available separately) to efficiently extract and solubilize crosslinked DNA-protein interaction complexes from cells, plus an immunoprecipitation module that contains high-capacity, ChIP-grade Protein A/G agarose.

Highlights:

• Simple and streamlined ChIP assay protocol (< 8 hours)
• Highly efficient isolation and lysis of nuclei
• Easy and reproducible enzymatic digestion
• Low-background and high-capacity Protein A/G agarose resin
• Highly specific positive control reagents included: RNA polymerase II antibody and GAPDH PCR primers
• Fast and reproducible spin-column format; columns and collection tubes included
• High-recovery DNA purification
• Choose a complete ChIP assay kit or the separate chromatin preparation module

Includes:

Kit contains the Chromatin Prep Module (Part No. 26158; 11 components) for chromatin extraction and a complete set of immunoprecipitation reagents (15 components) for IP experiments with controls

Specifications

• Binding Capacity: >50 mg Human IgG/mL resin binding capacity
• Capacity (Metric): ≥50 mg Human IgG per mL Resin Binding
• Description: Pierce Agarose ChIP Kit
• Content And Storage: Sufficient For: 30 complete chromatin IP assay experiments• ChIP Grade Protein A/G Plus Agarose, 0.65 mL (store at 4°C)
  • IP Dilution/Wash Buffer (5X), 11 mL (store at 4°C)
  • IP Wash Buffer 3 (5X), 4.5 mL (store at 4°C)
  • IP Elution Buffer (2X), 4.5 mL (store at 4°C)
  • Column Accessory Pack, 3 packs (store at room temperature or 4°C)
  • Microcentrifuge Tubes (1.5mL), 75 tubes (store at room temperature or 4°C)
  • DNA Clean-Up Column, 40 columns (store at room temperature or 4°C)
  • DNA Column Binding Solution, 30 mL (store at room temperature or 4°C)
  • DNA Column Wash Solution, 6 mL (store at room temperature or 4°C)
  • pH Indicator, 0.8 mL (store at room temperature or 4°C)
  • DNA Column Elution Solution, 5 mL (store at room temperature or 4°C)
  • Anti-RNA Polymerase II Antibody, 25 μL (store at -20°C)
  • Normal Rabbit IgG (1 mg/mL), 10 μL (store at -20°C)
  • ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL (store at -20°C)
  • Pierce Chromatin Prep Module (Part No. 26158) (store Proteinase K and Micrococcal Nuclease at -20°C. Store all other components at 4°C).
• Product Line: Pierce™
• Quantity: 30 Reaction Kit
• Type: ChIP Kit
• Sufficient For: 30 Reactions
• Format: Kit

Safety and Handling

Product Identifier

• Pierce Agarose ChIP Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H336-May cause drowsiness or dizziness.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Isopropyl alcohol, Guanidine hydrochloride, Sodium fluoride,Lithium chloride, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.