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Molecular Probes™ pHrodo™ Green Maleimide
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Quantity:
1 mg
Unit Size:
1mg
Description
The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
Use pH-sensitive pHrodo™ Green Maleimide to make pH-sensitive bioconjugates of your choice
Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others
The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.
pHrodo™ Green is also available in an amine-reactive form (see pHrodo™ Green STP), as well as a selection of ready-to-use conjugates (e.g., E. coli, S. aureus, and dextran). In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.
pHrodo™ Green is also available in an amine-reactive form (see pHrodo™ Green STP), as well as a selection of ready-to-use conjugates (e.g., E. coli, S. aureus, and dextran). In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Specifications
Specifications
| Format | Dried Down |
| Quantity | 1 mg |
| Product Type | Maleimide |
| Solubility | DMSO (Dimethylsulfoxide) |
| Content And Storage | 1 vial , store ≤ -20 °C, dessicate and protected from light. |
| Chemical Reactivity | Thiol |
| Detection Method | Fluorescence |
| EC Extinction Coefficient | 75000 |
| Reactive Group | Thiol |
| Label or Dye | pHrodo™ Green |
| Show More |
Safety and Handling
Product Identifier
- pHrodo Green Maleimide
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 1 B
- Skin sensitiser Category 1
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H317-May cause an allergic skin reaction.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P330-Rinse mouth.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 1H-Pyrrole-2,5-dione
For Research Use Only. Not for use in diagnostic procedures.
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