Thermo Scientific™ phi29 DNA Polymerase

Perform highly efficient isothermal DNA amplification with phi29 DNA Polymerase, a highly processive polymerase with strong strand displacement activity.

Brand: Thermo Scientific™

Manufacturer Part Number: EP0091

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg

Disclaimers: Use of this enzyme in certain applications may be covered by patents and may require a license.

Product Code. 10233130

Quantity Price
1 £ 56.25 / 250 units
EU Stock 21
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Description and Specification


Product Name phi29 DNA Polymerase
Quantity 2.5µg (250U)
Includes 1mL of 10X Reaction Buffer
Item Description 0.1ug/uL, (10ug/uL), 2.5ug (250U)
For Use With (Application) Rolling circle amplification (RCA) (6): generation of periodic DNA nanotemplates (7); Multiple displacement amplification (MDA) (8); Unbiased amplification of whole genome (WGA) Addition of Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase


  • Highest processivity and strand displacement activity among known DNA polymerases – more than 70 kb long DNA stretches can be synthesized
  • Highly accurate DNA synthesis (see Reference 5)
  • Extremely high yields of amplified DNA even from minute amounts of template
  • Amplification products can be directly used in downstream applications (PCR, restriction digestion, SNP genotyping, etc.)