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Invitrogen™ eBioscience™ Phalloidin eFluor™ 520

Product Code. 15590387
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Quantity:
300 tests
Unit Size:
300 tests
This item is not returnable. View return policy

Product Code. 15590387

Brand: Invitrogen™ 59655905

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This item is not returnable. View return policy

Description

Staining with Phalloidin eFluor™ 520 may be performed at the same time as fluorophore-conjugated antibodies. For best results visualize phalloidin-stained cells within 1 hour of staining.

Phalloidin is a peptide toxin isolated from the Amanita phalloides mushroom. Phalloidin selectively binds F-actin and functions to stablilize actin polymers by inhibiting the dissociation of actin monomers from the filament ends. Because F-actin polymers compose the cytoskeleton of almost all eukaryotic cells, phalloidin conjugates can be useful probes for visualizing the structure of live or fixed cells using microscopy. Phalloidin eFluor 520 can be used to label cells of multiple species. Its exitation and emission is similar to Alexa Fluor 488 and FITC.

Phalloidin eFluor 520 is supplied as a frozen liquid. To prepare the 1000X stock solution, thaw the vial, add 30μL high-quality anhydrous DMSO, mix and briefly spin down. The resulting solution (phalloidin DMSO stock) should be aliquoted, protected from light and moisture, and stored at -20°C. To prepare a 1X working solution of phalloidin conjugate, add 1μL of 1000X phalloidin DMSO stock solution to 1mL of PBS containing 1% BSA.

TRUSTED_SUSTAINABILITY

Specifications

Excitation Wavelength Range 488/520
Dye Type eFluor 520
No. of Tests 300 tests
Quantity 300 tests
Product Line eBioscience
Label Type eFluor
Product Type Phalloidin
SubCellular Localization Cytoskeleton

For Research Use Only. Not for use in diagnostic procedures.

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