missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. 17373919

DiscoverX PathHunter™ eXpress SYK Activity Assay

Product Code. 17373919
Encompass
Change view
Click to view available options
Quantity:
200 dp (2 x 96-well)
Unit Size:
1 set
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
17373919 200 dp (2 x 96-well) 1 set
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 17373919 Supplier DiscoverX Supplier No. 930865E3CP16M

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

The PathHunter™ eXpress SYK Activity Assay measures SYK CTK activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.

The PathHunter™ eXpress SYK Activity Assay provides an easy-to-use cell based assay to study small molecule drug candidates targeting CTKs. This kit assesses CTK activation via detection of resulting GHR phosphorylation-induced SH2 domain protein recruitment, mediated by SYK-JAK2 fusion protein. This product uses EFC technology. The included cells overexpress PK-tagged relevant cytokine receptor and EA-tagged SH2 domain protein alongside an engineered fusion of the cytokine receptor binding domain of JAK2 (Janus Kinase 2) to the catalytic domain from the CTK of interest, SYK. Upon activation of the cytokine receptor, the CRBD-CTK fusion phosphorylates the receptor, leading to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Specifications

Assay Entry SH2 recruitment
For Use With (Application) Assay-Ready eXpress Kits
Primary Agonist Recombinant Human Growth Hormone
Quantity 200 dp (2 x 96-well)
Target Entry SYK
Cell Line U2OS
Target SYK
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.