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DiscoverX PathHunter™ eXpress KDR Activity Assay

Product Code. 17333989
Encompass
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Quantity:
1,000 dp (10 x 96-well)
Unit Size:
1 set
This item is not returnable. View return policy

Product Code. 17333989

Brand: DiscoverX 930903E3CP16L

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This item is not returnable. View return policy

Description

The PathHunter™ eXpress KDR Activity Assay measures KDR receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.

The PathHunter™ KDR Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cells overexpress a PK-tagged engineered fusion of the catalytic domain of KDR to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Specifications

Assay Entry SH2 recruitment
For Use With (Application) Assay-Ready eXpress Kits
Format 1,000 dp (10 x 96-well)
Primary Agonist Recombinant Human EGF
Quantity 1,000 dp (10 x 96-well)
Target Entry KDR
Cell Line U2OS
Target KDR
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