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Invitrogen™ Ni-NTA Agarose
Description
Includes
The Ni-NTA resin is pre-charged and able to bind up to 50mg of recombinant protein per 1mL of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+
- Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
- Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
- One-step purification can be performed under both native and denaturing conditions
- Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications
- Guaranteed stable for 6 months when properly stored at 4°C
Specifications
Specifications
| Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
| Stationary Phase | Ni-NTA |
| Type | Agarose |
| Quantity | 100 mL |
| Form | Suspension |
| Column Type | Affinity Column |
Safety and Handling
Product Identifier
- Ni-NTA Agarose
- Warning
- Flammable liquid Category 3
- H226-Flammable liquid and vapour.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P240-Ground/bond container and receiving equipment.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P242-Use only non-sparking tools.
- P243-Take precautionary measures against static discharge.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contain: ethanol
For Research Use Only. Not for use in diagnostic procedures.
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