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NKG2A/C/E Monoclonal Antibody (20d5), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 11-5896-85
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The 20d5 monoclonal antibody reacts with the mouse NKG2A, C, and E. NKG2 molecules belong to a C-type lectin-like family of cell surface receptors expressed by mouse NK and NKT cell lineages. NKG2 molecules form heterodimeric complexes with CD94 and are responsible for recognition of non-classical MHC class I antigen Qa-1. When co-staining with NKG2A^B6 clone 16a11, it is important to stain for NKG2A-B6 first then subsequently with NKG2A/C/E, as steric hindrance has been observed if co-stained concurrently. Applications Reported: 20d5 has been reported for use in flow cytometric analysis. Applications Tested: The 20d5 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed.Specifications
NKG2A/C/E | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
0 | |
Klrc1, Klrc2, Klrc3 | |
Affinity chromatography | |
RUO | |
16641, 16642, 58179 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
20d5 | |
FITC | |
Klrc2 | |
CD159a; cd159c; cd159e; Cluster of Differentiation 159a; killer cell lectin-like receptor subfamily C, member 1; killer cell lectin-like receptor subfamily C, member 2; killer cell lectin-like receptor subfamily C, member 3; Klrc1; Klrc2; Klrc3; natural killer cell group 2E cell receptor; NKG2; NKG2A; NKG2-A/NKG2-B type II integral membrane protein; NKG2B; NKG2C; Nkg2e | |
Rat | |
500 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.