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CellSensor™ CellSensor™ NFκB-bla THP-1 Cell Line

Product Code. 10266743
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Quantity:
1 vial
Unit Size:
Each
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Product Code. 10266743

Brand: CellSensor™ K1662

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Description

CellSensor™ NFκB-bla THP-1 Cell Line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B response element.

  • The CellSensor™ NFκB-bla THP-1 cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B (NFκB) response element stably integrated into THP-1 cells.
  • To obtain the cell line, the NFκB-bla construct was transduced into THP-1 cells by lentivirus.
  • Subsequent flow cytometry was used to isolate cells responsive to Tumor Necrosis Factor alpha (TNFα).
  • The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, and EC50 concentration of TNFα.
  • The CellSensor™ NFκB-bla THP-1 cell line is responsive to TNFα and can be used to probe NFκB signaling pathways, including those involved in apoptosis, viral defense, cancer, inflammation, and autoimmune disease.
  • This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries.
  • Candidate drugs can be tested for dose response against this cell line.
TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Product Line CellSensor
Quantity 1 vial
Target Entry TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway
Type Cell Line
Cell Line THP-1
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

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