Nanog Monoclonal Antibody (eBioMLC-51), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5761-95

Product Code. 17130682

Description

The eBioMLC51 MAb recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells. Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved. Immunoblotting using eBioMLC51 reveals a band at ∽ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen. Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse Nanog.

Nanog Monoclonal antibody specifically detects Nanog in Mouse samples. It is validated for Immunocytochemistry, Immunofluorescence, Western Blot

Specifications

• Antigen: Nanog
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q80Z64
• Host Species: Rat
• Quantity: 10 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Clone: eBioMLC-51
• Conjugate: Unconjugated
• Gene: NANOG
• Gene Alias: 2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; Enk; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; homeodomain transcription factor Nanog; NANOG; Nanog homeobox; Nanog homeodomain transcription factor; nanog homeodox protein
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 71950
• Content And Storage: 4° C
• Form: Liquid