Nanog Antibody (5A10) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 6 publications

Brand:  Novus Biologicals NBP1-04320SS

Product Code. 18283981

Description

Nanog Monoclonal specifically detects Nanog in Human samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Nanog
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:500-1:1000, Flow Cytometry, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:100
• Gene Accession No.: Q9H9S0
• Gene Symbols: NANOG
• Immunogen: Recombinant human Nanog (1-154aa) purified from E. coli (NP_079141).
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Clone: 5A10
• Conjugate: Unconjugated
• Formulation: PBS (pH 7.4), 10% Glycerol with 0.02% Sodium Azide
• Gene Alias: FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
• Host Species: Mouse
• Molecular Weight of Antigen: 35 kDa
• Quantity: 0.025 mL
• Research Discipline: Embryonic Stem Cell Markers, Stem Cell Markers
• Gene ID (Entrez): 79923
• Target Species: Human, Mouse
• Form: Purified