Myogenin Mouse, Clone: SPM144, Novus Biologicals™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
18186942	Novus Biologicals NBP2-32987-0.02MG	0.02 mg
18137434	Novus Biologicals NBP2-32987-0.1MG	0.1 mg

Description

Myogenin Monoclonal specifically detects Myogenin in Human, Mouse, Rat, Porcine, Feline samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Myogenin
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Stem Cell Markers, Transcription Factors and Regulators
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: BHLHC3, bHLHc3Myogenic factor-4; myogenin, Class C basic helix-loop-helix protein 3, myf-4, MYF4myogenin, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)
• Gene Symbols: MYOG
• Isotype: IgG1 κ
• Purification Method: Protein A purified
• Test Specificity: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

• Clone: SPM144
• Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Feline
• Gene Accession No.: P15173
• Gene ID (Entrez): 4656
• Immunogen: Human myogenin recombinant protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 34 kDa