Invitrogen™ Mouse TNF alpha, Recombinant Protein

Product Code. 15587516

Brand: Invitrogen™ 14832162

Description

Recombinant Protein

Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4⁺ T cells and NK cells. A 26 kDa form of TNF-α is expressed as a membrane-bound molecule. TNF-α is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-α have been observed.

Specifications

• Accession Number: NM_013693
• For Use With (Application): Cytokine Bioassay
• Formulation: Sterile liquid; 10mM sodium phosphate, pH 7.2, 150mM NaCl, 0.5% BSA. 0.22μm filtered.
• Molecular Weight (g/mol): The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,258. The DTT-reduced and non-reduced protein migrate as 17kDa polypeptides on SDS-PAGE.
• Name: TNF alpha
• Purification Method: SDS-PAGE
• Quantity: 5 μg
• Source: E. coli
• Regulatory Status: RUO
• Structural Form: E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF- alpha accession # NM_013693
• Endotoxin Concentration: Less than 0.01ng/μg cytokine as determined by the LAL assay.
• Gene Symbol: Tumor Necrosis Factor alpha, TNFa TNF-a
• Biological Activity: The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10⁷ Units/mg.
• Conjugate: Unconjugated
• Cross Reactivity: Mouse
• Recombinant: Recombinant
• Purity or Quality Grade: >98%

For Research Use Only.