Store in freezer (-5° to -30°C) and protect from light.
Labeled calcium indicators are molecules that exhibit increase in fluorescence upon binding Ca2+. They have uses in many calcium signaling investigations, including measuring Ca2+ in cells and tissues that have high levels of autofluorescence and also for detecting Ca2+ release generated by photoreceptors and photoactivatable chelators.
Fluorescence signal from these cells is generally measured using fluorescence microscopy
Label (Ex/Em of Ca2+-bound form): Rhod-2 (552/581nm)
Fluorescence intensity increase upon binding Ca2+: >100 fold
Kd for Ca2+ in the absence of Mg2+, in buffer: approximately 570nM
Exhibit fluorescence increase upon binding Ca2+ with little shift in wavelength
In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn2+, Zn2+, Pb2+) with substantially higher affinity than Ca2+
Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN
Calcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling