Invitrogen™ Image-iT™ Lipid Peroxidation Kit, for live cell analysis

Enables detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent

Brand:  Invitrogen™ C10445

Product Code. 12877552

Includes: Five single-use vials with enough total reagent for five 96-well plates or 100 coverslips, and cumene hydroperoxide as a positive control

Description

This kit localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from approx. 590nm to approx. 510nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry.

• Includes cumene hydroperoxide as positive control compound to induce lipid peroxidation in cells
• Reagent is provided as stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• Form: Frozen

Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Nitro-Oxidative Stress, Reactive Oxygen Species

Order Info

Shipping Condition: Wet Ice

Specifications

• Color: Green
• Format: Frozen

Safety and Handling

Product Identifier

• Image-iT Lipid Peroxidation Kit, for live cell analysis

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• Aspiration toxicity Category 1
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Organic peroxide Type E & F
• Skin corrosion/irritation Category 1 B
• Specific target organ toxicity after repeated exposure Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H242-Heating may cause a fire.
• H302-Harmful if swallowed.
• H304-May be fatal if swallowed and enters airways.
• H312-Harmful in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H319-Causes serious eye irritation.
• H331-Toxic if inhaled.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.
• H373-May cause damage to organs.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Cumene hydroperoxide, Dimethyl sulfoxide

For Research Use Only. Not for use in diagnostic procedures.