Invitrogen™ EnzChek™ Phospholipase A1 Assay Kit

For continuous monitoring of phospholipase A1 (PLA1) in purified enzyme preparations, cell lysates and living cells

Marca:  Invitrogen™ E10219

Código de producto. 10627215

Includes: 1 kit; Sufficient material is supplied for 200 reactions in 96-well microplates at 100μL per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤25μL per well.

Descripción

This kit takes the stand-alone assay for phospholipase A1, PLA1 and combines the necessary reagents to run 2 to 10 complete 96-well microplate assays that monitor activity in purified enzyme preparations and cell lysates. The importance of phospholipases in cellular signaling, lipid metabolism, inflammatory responses and pathological disorders related to these processes has stimulated demand for fluorescence-based activity monitoring methods. In particular the phospholipases resident in plasma and endothelium can perturb circulating LDL and HDL particles, creating pro-artherogenic forms. Recent evidence is drawing a link between these lipases and the progression of several severe neurodegenerative diseases, including Alzheimer's. PLA-1 improves upon two existing reagents in two ways: first it is now PLA-1 specific and secondly it has improved the assay quality by decreasing initial background noise.

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

Información de órdenes

Shipping Condition: Room temperature

Especificaciones

• Content And Storage: 1 kit. Store kit at -<20°C, protected from light, desiccated
  
  Sufficient material is supplied for 200 reactions in 96 well microplates at 100 μl per well as described in the protocol below or 400 reaction in 384 low volume microplates at a volume of ≤ 25 μL per well.
• For Use With (Application): Phospholipase Assay
• Product Line: EnzChek™, Molecular Probes™
• Shipping Condition: Room Temperature
• Substrate Type: Phospholipase Substrate

• Detection Method: Fluorescence Intensity
• Product Type: Phospholipase Assay
• Quantity: 200 reactions, 200 Reactions
• Substrate Properties: Lipid-Based Substrate, Chemical Substrate
• Target Enzyme: Phospholipase

For Research Use Only. Not for use in diagnostic procedures.