Learn More
Invitrogen™ EnzChek™ Lysozyme Assay Kit
Description
Measure lysozyme activity in solution
The EnzChek™ Lysozyme Assay Kit provides researchers with a simple and sensitive assay to measure lysozyme activity in solution. The increase in fluorescence resulting from lysozyme activity is measured using a fluorometer or fluorescence microplate reader.
- Detect lysozyme activity concentrations as low as 20 U/mL
- Use standard fluorescein (FITC) excitation/emission settings
- Format allows for continuous measurement of lysozyme activity
The assay utilizes an innovative approach to measure lysozyme activity. Micrococcus lysodeikticus cell walls are used that have been extensively labeled with fluorescein causing quench of the fluorescence signal. Active lysozyme enzyme hydrolyzes the b-(1-4)-glucosidic linkages between the N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the mucopolysaccharide cell wall, relieving the quenching and yielding a dramatic increase in fluorescence that is proportional to lysozyme activity.
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Enzyme and Metabolic Activity |
| For Use With (Equipment) | Microplate Reader |
| Product Type | EnzChek Assay Kit |
| Product Line | EnzChek |
| Quantity | 400 Assays |
| Shipping Condition | Room Temperature |
| Substrate Properties | Lipid-Based Substrate |
| Substrate Type | Lysozyme Substrate |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.