Store in freezer (-5 to -30°C) and protect from light.
Free Radical Sensor
It is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.
Ex/Em of DAF-FM: approx. 495/515nm
Lyophilized product should be dissolved using DMSO and then added to an aqueous buffer to create a working solution
DAF-FM diacetate is cell permeant and passively diffuses across cellular membranes; once inside the cell, it is converted to cell-impermeant for
Buffers containing bovine serum albumin (BSA) and phenol red may affect fluorescence and should be used with caution
Fluorescence quantum yield of DAF-FM is approx. 0.005, but increases about 160-fold, to ~0.81, after reacting with NO
Assessment of NO production in transaldolase-deficient lymphoblasts by flow cytometry
Detection of NO accumulation in embryonic cortical neurons following neurotrophin stimulation
in vivo imaging of NO in zebrafish
ntravital microscopic detection of NO generation associated with angiogenesis in mice
Quantitation of ATP-induced NO release in rabbit platelets
Spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5
NO adduct of DAF-FM is significantly more photostable than that of DAF-2, which means additional time for image capture
DAF-FM is a more sensitive reagent for NO than is DAF-2 (NO detection limit for DAF-FM approx. 3nM versus approx. 5nM for DAF-2)
Refer to the Molecular Probes™ Handbook for additional product information.