Molecular Probes™ Click-iT™ EdU Alexa Fluor™ 594 HCS Assay

Superior alternative to traditional proliferation assays

Brand: Invitrogen

Manufacturer Part Number: C10355


UNSPSC: 41116113

Code: 50

Additional Details:
Additional Details: Weight: 0.00450kg

Product Code. 10258284

Quantity Price
1 £1688.00 / Each
Estimated Shipment date
from Supplier 28-10-2016
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Quantity 10 x 96 well plates
Storage Requirements Store at 2° to 6°C, dessicate and protect from light.
Detection Method Fluorescent
For Use With (Equipment) High Content Instrument
Kit Contents Sufficient material for 10 x 96-well plates
Product Type HCS (High Content Imaging) Assay
Sufficient For 10 x 96 well plates
Format 96-well plate
Excitation Wavelength Range 590/617
Dye Type Alexa Fluor 594
Color Red

Mild fixation and detergent permeabilization sufficient for Click-iT EdU detection reagent to gain access to the DNA. The Click-iT EdU Alexa Fluor 594 HCS Assay is a superior alternative to traditional proliferation assays that is optimized for high-content imaging applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor dye in a fast, highly specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol. The most accurate proliferation-detection methods are based on the incorporation and measurement of nucleoside analogues in newly synthesized DNA, with bromodeoxyuridine (BrdU) a commonly used analogue. BrdU-labeled DNA is quantitated using anti-BrdU antibodies following DNA denaturation by harsh methods (HCl, heat, or enzymes) to expose the BrdU molecules. This step is time consuming and difficult to perform consistently. The harsh treatment can also adversely effect sample integrity and quality, which makes co-staining with other antibodies challenging.

  • Simple: works the first time, every time, in less time
  • Efficient: no denaturation steps or harsh treatment required
  • Content-rich results: better preservation of cell morphology, antigen structure, and DNA integrity
  • Consistent: not dependent on variable antibody lots for detection
  • Less hazardous

Superior Proliferation Methodology
The Click-iT EdU Alexa Fluor 594 HCS Assay provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. With Click-iT EdU, mild fixation and detergent permeabilization is sufficient for the small molecule-based Click-iT EdU detection reagent to gain access to the DNA. As a consequence, the Click-iT EdU HCS assay is not only easy to use, but more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

The Click-iTassay can be used on cells in culture or in vivo following administration of EdU by feeding or injection methods.
The Click-iTassay can be used with BrdU in dual pulse experiments by using the ant-BrdU (clone MoBu-1) antibody, which does not cross react with EdU.
The Click-iTtechnology is compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed-cell labeling.

Cell Analysis, Cell Cycle, Cell Proliferation, Cell Viability, Proliferation and Function, Cellular Imaging, DNA Replication and Repair, High-Content Screening (HCS)