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Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay
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Product Code. 10074833 Shop All Thermo Scientific Products
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Quantity:
2 plates
Unit Size:
Each
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Product Code. 10074833

Brand: Invitrogen™ C10289

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380.00 GBP valid until 2025-12-19
BEST PRICE on Promo! Use promo code "24110" to get your promotional price.
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Includes

The kit contains sufficient reagents for performing two plates in a 96-well plate format.

Non-radioactive technique

The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or 'click' reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor 488 alkyne. The Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min/Max format.

  • Less hazardous

Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Color Blue, Green
Content And Storage The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  • Store at ≤-20°C, desiccate and protect from light.
    		•
    Detection Method Fluorescence
    For Use With (Application) High-content Screening (HCS)
    For Use With (Equipment) Fluorescence Microscope, High Content Analysis Instrument
    Product Type HCS Assay
    Dye Type Alexa Fluor™ 488, Hoechst 33258
    Format 96-well plate
    Green Features Less hazardous
    Product Line Alexa Fluor, Click-iT, Molecular Probes
    Quantity 2 plates
    Shipping Condition Room Temperature
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    For Research Use Only. Not for use in diagnostic procedures.

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