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Invitrogen™ CellROX™ Green Reagent, for oxidative stress detection
Description
Includes
Five single-use vials with enough total reagent for five 96-well plates or 100 coverslips
Cell-permeant dye is weakly fluorescent while in reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ∽485/520 nm. This reagent can be formaldehyde-fixed and its signal survives detergent treatment, which makes it multiplexable with other compatible dyes and antibodies.CellROXGreen Reagent is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS) as well as benchtop instruments such as FLoid, Tali, and Attune instruments.
- Compatible with other live cell dyes, antibodies, and in cells expressing RFP
- Provided as stable, ready-to-use DMSO solution with simple no-wash protocol compatible with standard workflows in fluorescence microscopy
- Oxidative stress results from an imbalance between production of ROS and ability of cells to scavenge them
- ROS play important role in the progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders
- Form: Frozen
- Excitation Class: Visible
Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Nitro-Oxidative Stress, Reactive Oxygen Species
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Color | Green |
| Format | Frozen |
| Quantity | 5 x 50 μL |
| Content And Storage | Store at ≤–20°C. Protect from light and desiccate. |
| For Use With (Equipment) | Imaging, HCS, Cytometer |
| Concentration | 2.5 mM stabilized solution in DMSO |
| Detection Method | Live Cell Imaging |
| Excitation/Emission | 485/520 nm |
| Indicator | Oxidative stress |
| Product Line | CellROX™ |
For Research Use Only. Not for use in diagnostic procedures.
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