Molecular Probes™ BCECF, AM (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein, Acetoxymethyl Ester)

For measuring changes in the cytosolic pH of most cells

Brand: Invitrogen

Manufacturer Part Number: B1170

X20 2',7'-Bis-(2-carboxyethyl)-5-( and-6)-carboxyfWith a pKa of ~6.98, the cell-permeant,

UNSPSC: 12161500

Code: 50

Additional Details:
Additional Details: Weight: 0.00525kg

Product Code. 11524147

Quantity Price
1 £ 207.0 / 1000µg
EU Stock 7
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


For Use With (Equipment) Microplate Reader
Quantity 20 x 5μg
Storage Requirements Store at room temperature and protect from light.

  • With pKa of approximately 6.98, cell-permeant, dual-excitation ratiometric pH indicator, AM is ideal for measuring changes in cytosolic pH of most cells
  • Intracellular pH measurements with BCECF are made by determining pH-dependent ratio of emission intensity (detected at 535nm) when dye is excited at approximately 490nm versus emission intensity when excited at its isobestic point of approximately 440nm

Cell Adhesion, Cell Analysis, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cell-Based Ion Channel Assays, Drug Discovery and Development, Ion Channel Biology, Ionic Homeostasis and Signaling, Target and Lead Identification and Validation, pH Detection