Molecular Probes™ Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

Brand:  Molecular Probes™ V35116

Product Code. 10494012

Description

This flow cytometry product provides a rapid and convenient assay for two hallmarks of apoptosis - phosphatidylserine externalization and changes in mitochondrial membrane potential. After staining a cell population with Alexa Fluor™ 488 annexin V and MitoTracker™ Red CMXRos dye in the provided binding buffer, live cells exhibit very little green fluorescence and bright red fluorescence, whereas apoptotic cells exhibit green fluorescence and decreased red fluorescence. These populations can easily be distinguished using a flow cytometer, and the 488 nm line of an argon-ion laser can be used to excite both dyes.

View a selection guide for all apoptosis assays for flow cytometry.

Specifications

• Format: Tube, Slide
• Content And Storage: Contains 1 vial of annexin, Alexa Fluor™ 488 conjugate (250 μL), 3 vials of MitoTracker™ red (50 μg per vial), 1 bottle of annexin binding buffer (5X solution, 15 mL), and 1 vial of DMSO (100 μL).
  
  Store in refrigerator (2–8°C) and protect from light.
• Excitation/Emission: Mitotracker™ red 579⁄599, Alexa Fluor™ 488: 499⁄521
• Shipping Condition: Wet Ice
• For Use With (Application): Flow Cytometry
• For Use With (Equipment): Fluorescence Microscope, Flow Cytometer

• Product Type: Mitochondrial Membrane Apoptosis Kit
• Flow Cytometer Laser Lines: 488
• No. of Reactions: 50
• Product Line: Alexa Fluor™, MitoTracker™
• Conjugate: Alexa Fluor 488, MitoTracker Red

For Research Use Only. Not for use in diagnostic procedures.