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MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), eFluor™ 450, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-5958-82
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed. Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.
MHC Class I (H-2Kb) Monoclonal antibody specifically detects MHC Class I (H-2Kb) in Mouse samples. It is validated for Flow Cytometry.Specifications
MHC Class I (H-2Kb) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01901, P03991, P04223, P14428 | |
H2-K1 | |
Affinity chromatography | |
RUO | |
14972 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
AF6-88.5.5.3 | |
eFluor 450 | |
H2-K1 | |
class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K1; histocompatibility 2, K1, K region; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen | |
Mouse | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.