MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15391810	Invitrogen 48-5958-80	25 μg
15301820	Invitrogen™ 48-5958-82	100 μg

Description

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed. Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

Specifications

• Antigen: MHC Class I (H-2Kb)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 14972
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: AF6-88.5.5.3
• Applications: Flow Cytometry
• Conjugate: eFluor 450
• Host Species: Mouse
• Target Species: Mouse
• Gene Symbols: H2-K1
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: H2-K1

For Research Use Only.