Invitrogen™ MAVS Monoclonal Antibody (ABM28H9), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen™ 17-9835-41

Product Code. 17191672

Description

Description: This ABM28H9 monoclonal antibody recognizes human MAVS, a mitochondrial membrane protein also known as VISA, CARDIF or IPS-1. Applications Reported: This ABM18H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This ABM18H9 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of HEL cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins located at www.thermofisher.com/flowprotocols. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. MAVS is a mitochondrial membrane protein that was identified as a critical component in the IFN beta signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappa-B. MAVS is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. MAVS also interacts with the IKK-alpha, IKK-beta and IKK-iota kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. Multiple isoforms of MAVS are known to exist.

Specifications

• Antigen: MAVS
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q7Z434
• Gene Symbols: MAVS
• Immunogen: Recombinant fragment of human MAVS (aa 72-296).
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: ABM28H9
• Conjugate: APC
• Gene: MAVS
• Gene Alias: CARD adapter inducing interferon beta; CARD adaptor inducing IFN-beta; Cardif; D430028G21Rik; IFN-B promoter stimulator 1; IFN-beta promoter stimulator-1; interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS1; IPS-1; KIAA1271; MAVS; mitochondrial antiviral signaling protein; mitochondrial anti-viral signaling protein; mitochondrial antiviral-signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adapter; virus-induced signaling adaptor; virus-induced-signaling adapter; VISA
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 57506
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid