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Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Blue/Red

Determine the viability of cells quickly and easily. Invitrogen™ ReadyProbes™ Cell Viability Imaging Kit, Red/Blue is a ready-to-use assay kit for use in for fluorescence microscopy, fluorescence microplate readers, and flow cytometry.

Brand:  Invitrogen™ R37610

192.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : NEW

Additional Details : Weight : 0.25000kg

Product Code. 15450054

  • £288.00 / 1 set
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Includes: 3 x 2.5mL NucBlue Live, 3 x 2.5mL propidium iodide

Description

Description

ReadyProbes™ Cell Viability Imaging Kit, Red/Blue contains NucBlue™ Live reagent (Hoechst 33342) and propidium iodide. Just add 2 drops of each stable reagent (at room temperature) to 1mL of cell growth media, then determine viability by counting total vs dead cells. NucBlue™ Live reagent stains the nuclei of all the cells and can be detected using a standard DAPI filter. Propidium iodide stains only the nuclei of cells with compromised plasma membrane integrity and is detected with a standard TRITC/RFP (orange) filter set.

  • NucBlue™ Live reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 360/460nm)
  • Propidium iodide: stains the nuclei of dead cells with compromised plasma membrane; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617nm)

Suggestions for use

  • NucBlue™ Live reagent and propidium iodide can be added directly to cells in full growth media or a compatible buffer solution.
  • In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
Specifications

Specifications

Mammalian Cells, Eukaryotic Cells
Store at 2° to 8°C, or at room temperature.
Fluorescence
Fluorescence Microscope
3 x 2.5 mL NucBlue™ Live; 3 x 2.5 mL propidium iodide
360, 535 nm
ReadyProbes
Blue, Red
ReadyProbes Cell Viability Imaging Kit, Blue/Red
Viability Assay
Cell Viability Imaging Kit
460, 617 nm
Dropper bottles
Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.