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Thermo Scientific™ LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit

Product Code. 13463269
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Quantity:
1 Kit
Unit Size:
1 set
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Product Code. 13463269

Brand: Thermo Scientific™ A14686

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Description

The LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit combines the flexibility of the BacMam gene delivery system with the robustness and power of our LanthaScreen™ TR-FRET technology.

The LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit combines the flexibility of the BacMam gene delivery system with the robustness and power of our LanthaScreen™ TR-FRET technology. It allows researchers to interrogate phosphorylation at serine 935 on a disease-relevant mutant (G2019S) Leucine-Rich Repeat Kinase-2 (LRRK2) protein in a variety of cell backgrounds to identify LRRK2 kinase inhibitors.

This assay format provides a convenient tool for the expression of G2019S LRRK2-GFP fusion protein in the cell background of your choice. The resulting cellular assay can be used to identify LRRK2 G2019S inhibitors, aiding in Parkinson's disease and other research focuses.

This kit provides enough material to evaluate the technology in a 384-well plate; larger sizes of all reagents are available separately.

The kit includes:

  • LanthaScreen™ Tb-anti-LRRK2 [pSer935] Antibody, 5 μg
  • BacMam LRRK2-GFP G2019S Reagent, 5 x 1 mL
  • LanthaScreen™ 6X Lysis Buffer, 6 mL
  • Instrument Control Terbium TR-FRET kit

A kit for interrogating wild-type LRRK2 protein (Cat. No. A14685) is also available.

Benefits of this assay include:

  • More Physiologically Relevant: cell-based LRRK2 kinase activity and choice of cell background
  • More Convenient: compatible with High Throughput Screening (HTS); LanthaScreen™ technology reduces interference and noise
  • Disease Relevant: screen compound libraries for Parkinson's disease-relevant molecules

More Physiologically Relevant

The LanthaScreen™ Tb-Anti-LRRK2 [pSer935] Antibody allows the investigation of phosphorylation at serine 935 on LRRK2 protein kinase. When paired with LRRK2 BacMam reagents, you have the freedom to choose the cellular background for your assay, including primary cells. This enables screening for potential inhibitors of LRRK2 proteins in their natural complexes in a physiologically relevant cell type.

More Convenient

Assays are run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as western blotting and ELISA, making this assay ideal for HTS applications. The LanthaScreen™ technology provides all of the advantages of TR-FRET ratiometric detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, which results in the use of fewer cells than traditional Western or ELISA methods.

Disease Relevant

The LanthaScreen™ Tb-Anti-LRRK2 [pSer935] Antibody can be used by Parkinson's and other disease researchers to perform HTS, cell-based assays to generate inhibition curves (IC50) for their compound libraries. This will aid drug discovery efforts by reducing the time to results from 4 hrs to typically 1.5 hrs compared to alternative ELISAs.

TRUSTED_SUSTAINABILITY

Specifications

Assay Cell-Based Assay
Assay Entry Cell-based terbium modification activity (transient)
Cell State Cells Not Included
Content And Storage • BacMam LRRK2-GFP G2019S, 5 x 1 mL (Store at 4°C)
• LanthaScreen™ 6X Lysis Buffer, 1 tube (Store at 4 °C)
• LanthaScreen™ Tb-anti-LRRK2 [pSer935] Antibody, 1 tube (Store at -20°C)
• Instrument Control Tb TR-FRET Kit (Store at 4°C)
• LanthaScreen™ LRRK2 [pSer935] G2019S Cellular Asasy Kit Protocol (Store at room temperature)
Description LanthaScreen™ LRRK2 G2019S [pSer935] Cellular Assay Kit, 1 Kit, Fluorescent Detection Method, Kinases Druggable Target, LanthaScreen™ System, TR-FRET Readout, Room Temperature Storage
Detection Method Fluorescence
For Use With (Application) TR-FRET
For Use With (Equipment) Microplate Reader
Includes 5 tubes BacMam LRRK2-GFP G2019S 1mL, Store at 4°C, 1 tube LanthaScreen™ 6X Lysis Buffer, Store at 4°C, 1 Tube LanthaScreen™ Tb-anti-LRRK2 [pSer935] Antibody, Store at –20°C, 1 Instrument Control Tb TR-FRET Kit, Store at 4°C,
Product Line LanthaScreen™
Quantity 1 Kit
Readout TR-FRET (Activity)
Reporter Gene GFP (EmGFP)
Target Entry LRRK2 G2019S
Type Cellular Assay Kit
System Type LanthaScreen™
Druggable Target Kinases
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For Research Use Only. Not for use in diagnostic procedures.

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