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Thermo Scientific™ LanthaScreen™ c-Jun (1-79) HeLa Cell Line
Description
The LanthaScreen™ c-Jun (1-79) HeLa cell line contains a fusion protein consisting of GFP and a fragment encoding for AA 1-79 of c-Jun under the control of a CMV promoter stably transfected into HeLa cell lines. HeLa is a cervical cancer cell line which shows inducible activation of JNK by a number of different ligands, such as TNF-a and EGF, which leads to the transient phosphorylation of GFP-c-Jun (1-79). This LanthaScreen™ cell line therefore allows the analysis of JNK activity using GFP-c-Jun phosphorylation as readout. The GFP-c-Jun (1-79) construct was transfected into HeLa cells using Lipofectamine™ 2000, followed by selection with Blasticidin. This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker. This cell line is validated for EC50 and Z' under optimized conditions using TNF-a as ligand for JNK mediated GFP-c-Jun (1-79) phosphorylation. This cell lines has also been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time. Additional information using alternate stimuli is also available.
Academic and non-profit customers, please inquire for special pricing.
Specifications
Specifications
| Assay Entry | Cell-based terbium phospho-specific activity (stable) |
| Cell State | Dividing Cells |
| Content And Storage | LanthaScreen™ Cellular assays are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use. |
| For Use With (Application) | Kinases, Signaling Pathway |
| Product Line | LanthaScreen |
| Quantity | 1 Vial |
| Target Entry | c-Jun |
| Type | Dividing Cells |
| System Type | LanthaScreen™ |
| Druggable Target | Signaling Pathway |
For Research Use Only. Not for use in diagnostic procedures.
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