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Alexa Fluor™ Kinase Tracer 314, 25 μM

Product Code. 10458468
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Quantity:
25 μL
Unit Size:
Each
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For Research Use Only. All usage must comply with product instructions.
 

Product Code. 10458468

Brand: Alexa Fluor™ PV6087

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Alexa Fluor™ Kinase Tracer 315 is detected by addition of a Eu-labeled anti-tag antibody.

  • Excitation/Emission (nm): 340/615, 665
  • Label or Dye: Alexa Fluor™ 647
  • Target Enzyme: Kinases (Multiple)
  • Validated Kinases: PIK3CD, PIK3CG, FRAP1 (mTOR)
  • In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
TRUSTED_SUSTAINABILITY

Specifications

Assay Kinase Binding Assay
Assay Entry Biochemical binding/kinetic
Content And Storage Store at -20°C.
Excitation/Emission 340/615, 665
For Use With (Application) TR-FRET
For Use With (Equipment) Microplate Reader
Format 384-well Plate
Label or Dye Alexa Fluor™ 647
Quantity 25 μL
Readout Kinetic
Shipping Condition Dry Ice
Target Entry PIK3CG, PIK3CD/PIK3R1, mTOR, FRAP1
Type Kinase Tracer
Validated Kinases PIK3CD, PIK3CG, FRAP1 (mTOR)
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For Research Use Only. Not for use in diagnostic procedures.

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