Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ TOP10F' Chemically Competent E. coli, no manual

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Includes: Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Product Code	Brand	Quantity
10593853	Invitrogen™ KNM455040	50 Reactions
10583853	Invitrogen™ KNM455001	25 Reactions

Description

Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.

• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• TOPO TA Cloning Kits are also available in combo kits combined with a PureLink™Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis

Cloning, PCR Cloning

Specifications

• Product Type: Kit, Cloning
• Includes: Box 1: Topoisomerase I-activated pCR2.1-TOPO vector, PCR buffer, Salt solution, dNTPs, Control template, M13 forward and reverse primers, Control PCR primers, Sterile water. Box 2: One Shot Chemically Competent or Electrocomp E. coli, S.O.C. medium, Supercoiled pUC19 control plasmid
• Bacterial or Yeast Strain: TOP10F´
• For Use With (Application): Cloning, PCR Cloning

For Research Use Only. Not for use in diagnostic procedures.