Invitrogen™ pBAD TOPO™ TA Expression Kit

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

Brand:  Invitrogen™ K430001

Product Code. 10115092

Description

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
• araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Promoter: araBAD
• Selection Agent (Eukaryotic): None
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Cleavage: EK (Enterokinase) Recognition Site
• Expression Mechanism: Cell-Based Expression
• Cloning Method: TOPO-TA
• Vector: pBAD

• Inducing Agent: Arabinose
• Product Type: TA Expression Kit
• Content And Storage: Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194 E. coli stab at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.
• Bacterial or Yeast Strain: LMG194, TOP10
• Expression System: E. coli
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Quantity: 20 Reactions
• Product Line: TOPO™

For Research Use Only. Not for use in diagnostic procedures.