Invitrogen™ pBAD TOPO™ TA Expression Kit

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

Brand:  Invitrogen™ K430001

Product Code. 10115092

Description

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
• araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Constitutive or Inducible System: Inducible
• Promoter: araBAD
• Selection Agent (Eukaryotic): None
• Bacterial or Yeast Strain: LMG194, TOP10
• Expression System: E. coli
• Protein Tag: His Tag (6x), V5 Epitope Tag
• Quantity: 20 Reactions
• Product Line: TOPO™

• Inducing Agent: Arabinose
• Product Type: TA Expression Kit
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Cleavage: EK (Enterokinase) Recognition Site
• Expression Mechanism: Cell-Based Expression
• Cloning Method: TOPO™-TA
• Vector: pBAD

For Research Use Only. Not for use in diagnostic procedures.