missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ pBAD TOPO™ TA Expression Kit

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

£992.00 - £1622.00

Specifications

Constitutive or Inducible System Inducible
Inducing Agent Arabinose
Promoter araBAD
Product Type TA Expression Kit
Selection Agent (Eukaryotic) None
View More Specs

Includes: Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Products 2
Product Code Brand Protein Tag Quantity Price Quantity & Availability  
Product Code Brand Protein Tag Quantity Price Quantity & Availability  
10414592
View Documents Promotion Details
Invitrogen™
K430040
V5 Epitope Tag 40 Reactions
£1622.00
Each
View alternative products
Save Up to 
»
Estimated Shipment: 05-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
10115092
View Documents Promotion Details
Invitrogen™
K430001
His Tag (6x), V5 Epitope Tag 20 Reactions
£992.00
Each
View alternative products
Save Up to 
»
Estimated Shipment: 22-04-2024
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

  • Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
  • araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
  • C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Specifications

Inducible
araBAD
None
LMG194, TOP10
E. coli
TOPO-TA
TOPO™
Arabinose
TA Expression Kit
Ampicillin (AmpR)
EK (Enterokinase) Recognition Site
Cell-Based Expression
pBAD
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.