IL-8 (1-77) (CXCL8), clone: N11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7189-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody N11 reacts specifically with the 77 amino acid form of IL-8 (IL-877, IL-8(1-77), CXCL877) and does not react with the more common 72 amino acid form. Frequently referred to as endothelial IL-8, the 77 amino acid form is secreted in cultured leukocytes. The 72 amino acid form is the result of efficient cleavage of an N-terminal pentamer from IL-877 after secretion. IL-8 is a C-X-C chemokine with potency in neutrophil chemotaxis and activation. IL-877 has decreased chemotactic activity when compared to IL-872, but has other specific pro-apoptotic and tumor cell chemotactic activities. IL-877 has recently been shown to be the major isoform present in premature neonates due to deficiency in serum protease activity that is not seen in term neonates.Applications Reported: This N11 antibody has been reported for use in immunoblotting (WB).Applications Tested: This N11 antibody has been tested by immunoblot of peripheral blood mononuclear cells stimulated with LPS for 4 hours. This can be used at 1-5 μg per ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|