IL-8 (1-77) (CXCL8) Monoclonal Antibody (N11), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 14-7189-80

Product Code. 15278037

Description

Description: The monoclonal antibody N11 reacts specifically with the 77 amino acid form of IL-8 (IL-877, IL-8(1-77), CXCL877) and does not react with the more common 72 amino acid form. Frequently referred to as endothelial IL-8, the 77 amino acid form is secreted in cultured leukocytes. The 72 amino acid form is the result of efficient cleavage of an N-terminal pentamer from IL-877 after secretion. IL-8 is a C-X-C chemokine with potency in neutrophil chemotaxis and activation. IL-877 has decreased chemotactic activity when compared to IL-872, but has other specific pro-apoptotic and tumor cell chemotactic activities. IL-877 has recently been shown to be the major isoform present in premature neonates due to deficiency in serum protease activity that is not seen in term neonates. Applications Reported: This N11 antibody has been reported for use in immunoblotting (WB). Applications Tested: This N11 antibody has been tested by immunoblot of peripheral blood mononuclear cells stimulated with LPS for 4 hours. This can be used at 1-5 μg per mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.

Specifications

• Antigen: IL-8 (1-77) (CXCL8)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P10145
• Gene Symbols: CXCL8
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3576
• Content And Storage: 4° C
• Form: Liquid

• Applications: Western Blot
• Clone: N11
• Conjugate: Unconjugated
• Gene: CXCL8
• Gene Alias: alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; granulocyte chemotactic protein 1; interleukin 8; interleukin-8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; lymphocyte-derived neutrophil-activating factor; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.