Invitrogen™ IL-4 Monoclonal Antibody (MP4-25D2), Functional Grade, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15537806	Invitrogen™ 16-7048-38	5 mg
17161462	Affymetrix eBioscience 16-7048-025	25 mg
17171462	Affymetrix eBioscience 16-7048-050	50 mg

Description

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells. Applications Reported: MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Applications Tested: The MP4-25D2 antibody has been tested for neutralizing IL-4 binding and biological activity. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Antigen: IL-4
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 3565
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Il4

• Clone: MP4-25D2
• Applications: Functional Assay, Neutralization
• Conjugate: Functional Grade
• Host Species: Rat
• Target Species: Human
• Gene Accession No.: P05112
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody