PeproTech IL-16 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17854283	PeproTech 500-P06BT-25UG	25 μg
17874363	PeproTech 500-P06BT-50UG	50 μg
17834433	Invitrogen 500-P06BT-1MG	2 x 500μg
30054468	PeproTech 500-P06BT-500UG	500 μg

Description

AA Sequence of recombinant protein: PDLNSSTDSA ASASAASDVS VESTAEATVC TVTLEKMSAG LGFSLEGGKG SLHGDKPLTI NRIFKGAASE QSETVQPGDE ILQLGGTAMQ GLTRFEAWNI IKALPDGPVT IVIRRKSLQS KETTAAGDS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-16. Anti-Human IL-16-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-16 (500-P06) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P06BT-1MG will be provided as 2 x 500 μg

The protein encoded by IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of IL-16 undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.

Specifications

• Antigen: IL-16
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q14005
• Gene ID (Entrez): 3603
• Immunogen: E.coli-derived Recombinant Human IL-16 (129 a.a.).
• Purification Method: Antigen affinity chromatography
• Gene: IL16

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: FLJ16806; FLJ42735; FLJ44234; H-IL-16; il 16; Il16; IL-16; ILN; Interleukin; interleukin 16; Interleukin16; Interleukin-16; LCF; Lymphocyte chemoattractant factor; M-IL-16; mKIAA4048; neuronal interleukin 16; NIL16; PRIL16; prIL-16; prointerleukin 16; Pro-interleukin-16
• Gene Symbols: IL16
• Primary or Secondary: Primary
• Content And Storage: -20°C