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Molecular Probes™ BacLight™ Bacterial Membrane Potential Kit

Product Code. 10730964
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For Research Use Only. All usage must comply with product instructions.
 
Product Code. 10730964 Supplier Molecular Probes™ Supplier No. B34950

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

BacLight™ Bacterial Membrane Potential Kit

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Contains DiOC2(3) (1.2 mL of 3 mM solution in DMSO), CCCP (300 μL of 500 μM solution in DMSO), and PBS (200 mL). Store in refrigerator (2–8°C) and protect from light.
Detection Method Fluorescence
For Use With (Application) Membrane Potential Assay
For Use With (Equipment) Flow Cytometer
Product Type Bacterial Membrane Potential Kit
Dye Type Membrane Potential Probes
Emission 482/497
Format Tube(s)
Product Line BacLight
Quantity 1 kit
Shipping Condition Wet Ice
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For Research Use Only. Not for use in diagnostic procedures.

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