eBioscience Human PEI-Transferrinfection

Nucleic acid delivery system based on transferrin receptor-mediated endocytosis to carry DNA into cells and the gene transfer efficiency of PEI/DNA complexes

Overview
Brand: eBioscience

Manufacturer Part Number: BMS1003/a

16RXN Human PEI-Transferrinfection 16 transferrinfections

Code: NEW

Additional Details:
Additional Details: Weight: 0.47500kg



Includes: Transferrin-Polyethylenimine-conjugate, lyophilized, 1 mg/mL upon reconstitution; iron incorporated
Deferrioxamine, lyophilied, 50 mM upon reconstitution
HBS buffer concentrate (20x)
(400mM Hepes, pH 7.3, 3M NaCl)

Product Code. 15541307

Quantity Price
1 £ 151.3 / 16 reactions
Estimated Shipment date
from Supplier 13-12-2016
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Description and Specification

Specification

For Use With (Application) Cell Culture
Quantity 16 transferrinfections
Species Human

  • Method results in a 30 to 1000-fold enhanced transfection efficiency depending on the cell line
  • An extremely gentle DNA transfection method which employs physiological uptake mechanisms of the cell
  • Transfection efficacy depends on the cell type, the level of surface transferrin receptor expression
  • Very high transfection rates have been shown for the tested tumor cell lines B16F10 melanoma, Neuro 2A neuroblastoma and a variety of primary human melanoma cell lines
  • In other established cell lines, such as HeLa, CHO, Jurkat, K562, HepG2 and COS the PEI-Transferrinfection works with high efficiences, excellent reproducibility and with the advantage of being an extremely gentle procedure