Gibco™ Human Noggin Recombinant Protein, PeproTech®

Product Code.	Quantity	unitSize
17840123	100 μg	100µg
17880143	2 x 500 μg	1mg
30087766	2 x 500 μg	Pack of 2
17811063	20 μg	20µg
17830873	250 μg	250µg
17850883	500 μg	500µg
17850123	50 μg	50µg
17861183	5 μg	5µg

Product Code. 17840123 Supplier Gibco™ Supplier No. 12010C100UG

Beschreibung

Recombinant Protein

120-10C-1MG will be provided as 2 x 500 μg (120-10C-500UG). Recombinant Human Noggin is a 46 kDa disulfide-linked homodimer consisting of two 205 amino acid polypeptide chains. Monomeric glycosylated noggin migrates at an apparent molecular weight of approximately 28.0-33.0 kDa by SDS PAGE analysis under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.

Spezifikation

• Accession Number: Q13253
• For Use With (Application): Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 9241
• Molecular Weight (g/mol): 46 kDa
• Name: Human Noggin
• Purification Method: Purified
• Quantity: 100 μg
• Source: HEK293 cells
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: NOG; Noggin; Noggin precursor; noggin; LOW QUALITY PROTEIN: noggin; SYM1; symphalangism 1 (proximal); SYNS1; SYNS1A
• Common Name: Noggin
• Gene Symbol: NOG
• Biological Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
• Content And Storage: -20°C
• Activity: Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.