Thermo Scientific™ Pierce™ Human IFN alpha ELISA Kits (Multi-Subtype)

Measure levels of 14 identified subtypes of human interferon alpha (IFNa) in human cell culture media supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ 411052

Product Code. 10167923

Description

Assay IFN-a (14 out of 15 identified subtypes of human interferon alpha) levels in human cell culture media with these VeriKine Human IFN alpha ELISA Kits (Multi-Subtype).

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100μL per well. These IFN-α kits are validated for use with human cell and tissue culture media and are optimized for both high-sensitivity and extended assay ranges. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

• Target: 14 of 15 subtypes of human interferon alpha (IFN-alpha): IFN-αA, IFN-α2, IFN-αD, IFN-αB2, IFN-αC, IFN-αG, IFN-αH, IFN-αI, IFN-αJ1, IFN-αK, IFN-α1, IFN-α4a, IFN-α4b, and IFN-αWA

• Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate

• Measurement: Absorbance at 450nm

• Assay Range: 12.5 to 500pg/mL (high sensitivity); 156 to 5000pg/mL (extended range)

• Sensitivity: Undetermined

• Precision: Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%

• Sample Types: Cell culture supernatant, tissue culture media

• Total Assay Time: 3.25 hours

• Sample Size: 100μL

• Specificity: No significant cross-reaction with human IFN-β, IFN-γ or IFN-ω; mouse or rat IFN-α, IFN-β, or IFN-γ; bovine IFN-τ

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.

Specifications

• Assay Range: High Sensitivity: 12.5 to 500pg/mL; Extended Range: 156 to 5000pg/mL
• Description: Human IFN alpha ELISA Kit (Multi-Subtype)
• Sample Type: Cell Culture Supernatant, Tissue Culture Media
• Sufficient For: 5 x 96 microplate wells
• Measurement: Absorbance at 450nm
• Quantity: 5 Plate Kit
• Target: 14 of 15 subtypes of human interferon alpha (IFN-alpha): IFN-aA, IFN-a2, IFN-aD, IFN-aB2, IFN-aC, IFN-aG, IFN-aH, IFN-aI, IFN-aJ1, IFN-aK, IFN-a1, IFN-a4a, IFN-a4b, and IFN-aWA

• Assay Sensitivity: Undetermined
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Specificity: No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
• Kit Contents: Antibody Concentrate, 1 vial, Dilution Buffer, 250mL, HRP Conjugate Concentrate, 1 vial, Wash Solution Concentrate, 250mL, TMB Substrate Solution, 60mL, Stop Solution, 60mL, Plate Sealers, 20 ea, HRP Conjugate Diluent, 60mL, Precoated 96-well Plates, 5 ea, Human IFN Alpha Standard 10,000pg/mL, 2.5mL
• Precision: Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
• Sample Volume: 100 μL
• Total Assay Time: 3.25 hr.