Thermo Scientific™ Pierce™ Pierce™ Human IFN alpha ELISA Kits (Multi-Subtype)

Measure levels of 14 identified subtypes of human interferon alpha (IFNa) in human cell culture media supernatant samples using 96-well plates.

Overview
Brand: Thermo Scientific™ Pierce™

Manufacturer Part Number: 411052

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10167923

Quantity Price
1 £ 1954.0 / 1 set
Estimated Shipment date
from Supplier 07-04-2017
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Description and Specification

Specification

Precision Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
Sensitivity Undetermined
Formulation Complete ELISA kit with coated 96-well plates
Measurement Absorbance at 450nm
Kit Contents Antibody Concentrate, 1 vial
Assay Range High Sensitivity: 12.5 to 500pg/mL; Extended Range: 156 to 5000pg/mL
Sufficient For 5 x 96 microplate wells
Format Colorimetric ELISA (TMB substrate) in clear 96-well plate
Specificity No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
Sample Type Cell culture supernatant, tissue culture media
Target 14 of 15 subtypes of human interferon alpha (IFN-alpha): IFN-aA, IFN-a2, IFN-aD, IFN-aB2, IFN-aC, IFN-aG, IFN-aH, IFN-aI, IFN-aJ1, IFN-aK, IFN-a1, IFN-a4a, IFN-a4b, and IFN-aWA
Sample Size 100µL
Description Human IFN alpha ELISA Kit (Multi-Subtype)
Total Assay Time 3.25 hr.
Quantity 5 plate kit

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100μL per well. These IFN-α kits are validated for use with human cell and tissue culture media and are optimized for both high-sensitivity and extended assay ranges. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: 14 of 15 subtypes of human interferon alpha (IFN-alpha): IFN-αA, IFN-α2, IFN-αD, IFN-αB2, IFN-αC, IFN-αG, IFN-αH, IFN-αI, IFN-αJ1, IFN-αK, IFN-α1, IFN-α4a, IFN-α4b, and IFN-αWA

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate

  • Measurement: Absorbance at 450nm

  • Assay Range: 12.5 to 500pg/mL (high sensitivity); 156 to 5000pg/mL (extended range)

  • Sensitivity: Undetermined

  • Precision: Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%

  • Sample Types: Cell culture supernatant, tissue culture media

  • Total Assay Time: 3.25 hours

  • Sample Size: 100μL

  • Specificity: No significant cross-reaction with human IFN-β, IFN-γ or IFN-ω; mouse or rat IFN-α, IFN-β, or IFN-γ; bovine IFN-τ

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.

Documentation

Technical documents