missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ Pierce™ Human IFN alpha ELISA Kits (Multi-Subtype)

Measure levels of 14 identified subtypes of human interferon alpha (IFNa) in human cell culture media supernatant samples using 96-well plates.

Brand:  Thermo Scientific™ Pierce™ 411052

UNSPSC : 41116126

Code : PN

Additional Details : Weight : 0.10000kg

 View more versions of this product

Product Code. 10167923

  • £1954.00 / 1 set


Estimated Shipment
12-10-2017
Add to basket

Description

Description

These human IFN alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100μL per well. These IFN-α kits are validated for use with human cell and tissue culture media and are optimized for both high-sensitivity and extended assay ranges. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: 14 of 15 subtypes of human interferon alpha (IFN-alpha): IFN-αA, IFN-α2, IFN-αD, IFN-αB2, IFN-αC, IFN-αG, IFN-αH, IFN-αI, IFN-αJ1, IFN-αK, IFN-α1, IFN-α4a, IFN-α4b, and IFN-αWA

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well plate

  • Measurement: Absorbance at 450nm

  • Assay Range: 12.5 to 500pg/mL (high sensitivity); 156 to 5000pg/mL (extended range)

  • Sensitivity: Undetermined

  • Precision: Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%

  • Sample Types: Cell culture supernatant, tissue culture media

  • Total Assay Time: 3.25 hours

  • Sample Size: 100μL

  • Specificity: No significant cross-reaction with human IFN-β, IFN-γ or IFN-ω; mouse or rat IFN-α, IFN-β, or IFN-γ; bovine IFN-τ

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.

Specifications

Specifications

High Sensitivity: 12.5 to 500pg/mL; Extended Range: 156 to 5000pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well plate
Antibody Concentrate, 1 vial
Absorbance at 450nm
Undetermined
100µL
5 x 96 microplate wells
14 of 15 subtypes of human interferon alpha (IFN-alpha): IFN-aA, IFN-a2, IFN-aD, IFN-aB2, IFN-aC, IFN-aG, IFN-aH, IFN-aI, IFN-aJ1, IFN-aK, IFN-a1, IFN-a4a, IFN-a4b, and IFN-aWA
Human IFN alpha ELISA Kit (Multi-Subtype)
Complete ELISA kit with coated 96-well plates
Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
5 plate kit
Cell culture supernatant, tissue culture media
No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
3.25 hr.
Documentation

Technical Documents