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Invitrogen™ Granzyme B Human ELISA Kit
For the quantitative detection of human Granzyme B.
£2545.00
Specifications
Accession Number | P10144 |
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Assay Range | 0.66-480 pg/mL |
Assay Sensitivity | 0.2 pg/mL |
Conjugate | Biotin |
Product Type | ELISA Kit |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15551517
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Invitrogen™
BMS2027TEN |
10 x 96 Tests |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
The Human Granzyme B ELISA quantitates Hu Granzyme B in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme B. Principle of the method The Human Granzyme B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
- Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders
- Not all granzymes enter the target cell, part of them also 'leak' in to the peripheral blood and other biological fluids
- Detectable amounts of granzymes have been found to circulate in healthy volunteers
- These soluble granzymes can be measured by ELISAs
Specifications
P10144 | |
0.2 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
C11,CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT | |
8.5% | |
HRP | |
Serum, 50 μL; Supernatant, 50 μL | |
Granzyme 2, Fragmentin 2, Human lymphocyte protein | |
1 hr. 20 min. |
0.66-480 pg/mL | |
Biotin | |
Serum, Supernatant | |
ELISA | |
3002 | |
10.4% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
RUO | |
2°C to 8°C | |
Human | |
2 hr. 45 min. |
For Research Use Only