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  6. Cytiva HisTrap™ FF Crude Prepacked Columns

Cytiva HisTrap™ FF Crude Prepacked Columns

Product Code. 10685355 Shop All Cytiva Products
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Quantity:
5 x 5 mL
60 mL
100 x 1 mL
100 x 5 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
Unit Size:
Pack of 100
Pack of 5
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Product Code. 10685355

Brand: Cytiva 11000458

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This item is not returnable. View return policy

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

TRUSTED_SUSTAINABILITY

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Description HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
Flow Rate <4 mL/min.
Kit Contents Sodium Phosphate-buffered Saline, pH 7.4 and 0.5X concentration of proprietary Y-PER reagent additives
Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 45 to 165 μm
Quantity 60 mL
For Use With (Application) Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
Type Pre-packed Column
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Safety and Handling

compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • EUH208-Contains . May produce an allergic reaction.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .
 Supplemental information
  • MIXTURE LIST-Contains : ethanol, Nickel
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