Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Product Code.	Quantity	unitSize
13404199	50 mL	50mL
13414199	1 L	1 L
13484189	10 mL	10mL
13494189	250 mL	250mL

Product Code. 13404199

Brand: Thermo Scientific™ 25215

Description

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Additional Information and Online Ordering

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

• High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

• High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

• Versatile – can be used to purify proteins under native and denaturing conditions

• Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

• Compatible – maintains function in a wide variety of chemicals and pH conditions

• Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Specifications

• Capacity (Metric): 20 mg His-Tagged GFP Binding per mL Resin
• Content And Storage: Store at 4°C
• Form: Liquid Suspension
• Product Line: HisPur
• Quantity: 50 mL
• Target: His-Tagged Protein
• Type: Agarose
• Stationary Phase: Divalent Nickel, Metal Chelate
• Format: Bottle
• Column Type: Affinity, Agarose Resin

Safety and Handling

Product Identifier

• HisPur Ni-NTA Superflow Agarose, 50 mL.

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Skin sensitiser Category 1
• Respiratory sensitiser Category 1
• Carcinogenicity Category 1
• Reproductive toxicity Category 1B
• Specific target organ toxicity after repeated exposure Category 2
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H350-May cause cancer.
• H360D-May damage the unborn child.
• H373-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P370+P378-In case of fire: Use to extinguish.

Supplemental information

• MIXTURE LIST-Contains : Ethyl alcohol, Nickel(II) sulfate hexahydrate(1:1:6)

For Research Use Only. Not for use in diagnostic procedures.