Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 0.2 mL

Product Code. 11802114

Brand: Thermo Scientific™ 88227

Description

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

• High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

• Versatile – purify proteins using native or denaturing conditions

• Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

• Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

• Cost-effective – reuse the same batch of resin at least five times

• Easy to use – pre-formulated buffers available for kit formats

Available Formats:

• Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
• Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
• Purification Kits – complete kits in all three column sizes
• Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
• Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns

Specifications

• Capacity (Metric): 0.2 mL/Column
• Description: HisPur Ni-NTA Spin Purification Kit, 0.2mL
• Content And Storage: Sufficient For: Purifying up to 12 mg of His-tagged protein per column
  • HisPur Ni-NTA Spin Columns, 0.2 mL, 25 columns
  • Phosphate-buffered Saline (10X), 30 mL
  • Imidazole (2M), 8 mL
  • Collection Tubes, 80 tubes
  • Column Plugs, 25 plugs
  
  Store at 4°C.
• Product Line: HisPur
• Quantity: 25 Column Kit
• Target: His-Tagged Protein
• Type: Spin Column Kit
• Stationary Phase: Divalent Nickel, Metal Chelate
• Sufficient For: 12 mg of His-Tagged Protein per Column
• Format: Kit, Microcentrifuge, Spin Column
• Column Type: Affinity, Agarose Resin

Safety and Handling

Product Identifier

• X25 HISPUR NI-NTA PURIFICATION KIT

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Carcinogenicity Category 1A
• Serious eye damage/eye irritation Category 2
• Reproductive toxicity Category 1B
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 1 B
• Skin sensitiser Category 1
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H350-May cause cancer.
• H360D-May damage the unborn child.
• H360FD-May damage fertility. May damage the unborn child.
• H373-May cause damage to organs.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P101-If medical advice is needed, have product container or label at hand.
• P102-Keep out of reach of children.
• P103-Read label before use.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Sulfuric acid, nickel(2+) salt, hydrate,sodium chloride, disodium hydrogenorthophosphate, imidazole

For Research Use Only. Not for use in diagnostic procedures.